PAHs Target Hematopoietic Linages in Bone Marrow through Cyp1b1 Primarily in Mesenchymal Stromal Cells but Not AhR: A Reconstituted In Vitro Model

Figure 2

Conditions that sustain progenitor colony forming activities in collagenase-released bone marrow cells (BMC). (a) Comparison of PreB CFU activity in BMC maintained alone in culture media for 24 hours after isolation (media) or sustained by being cocultured for this period with an adherent monolayer of either OP9 cells or BMS2 cells. Direct cultures were placed in the methocult media immediately upon BMC isolation. . (b) BMC PreB CFU activity following 24-hour culture in media alone, in coculture with OP9 cells, or with media enriched by 24-hour preculture with OP9 cells (OP9-EM).Statistical significance; value < 0.05. (c) CFU progenitor cell proliferative activity (PreB, granulocyte-monocyte (GM), and erythroid (E)) in BMC, when directly isolated or after 24 hours in OP9 coculture. for comparison between direct BMC culture and OP9 coculture.

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