PAHs Target Hematopoietic Linages in Bone Marrow through Cyp1b1 Primarily in Mesenchymal Stromal Cells but Not AhR: A Reconstituted In Vitro Model

Figure 5

Comparison of WT and Cyp1b1-ko BMC with respect to PreB CFU progenitor cell activity. (a) Comparison of PreB CFU activity from BMC immediately after isolation (direct) and after 24-hour OP9 coculture. (b) Comparison of the PreB CFU activities for BMC after 24-hour solvent control (DMSO) and DMBA (1 M) treatments, using OP9 coculture conditions. . (c) Comparison of the PreB CFU progenitor cell activity for WT and Cyp1b1-ko BMC after 24-hour solvent control (DMSO) and DMBA (1 M) treatments in OP9 enriched medium (OP9-EM).

(a)

(b)

(c)