PAHs Target Hematopoietic Linages in Bone Marrow through Cyp1b1 Primarily in Mesenchymal Stromal Cells but Not AhR: A Reconstituted In Vitro Model

Figure 6

Reversal of DMBA suppression of PreB CFU activity from BMC by Cyp1b1 inhibitors, a-naphthoflavone (a-NF) and tetramethoxystilbene (TMS). To enhance sensitivity to DMBA, BMC from XPC−/− mice (low DNA repair) are used for the OP9 coculture. DMBA concentration is then decreased to 0.05 M, which is more sensitive to inhibitors. Inhibitors (1 M) were incubated with the cells for 24 hours prior to PreB CFU analyses. .