PAHs Target Hematopoietic Linages in Bone Marrow through Cyp1b1 Primarily in Mesenchymal Stromal Cells but Not AhR: A Reconstituted In Vitro Model

Table 1

Genes targeted by Cyp1b1 endogenous substrates and AhR. Separation by DMBA responses.

Gene	WT (FC)		Cyp1b1-ko (FC)		Set
	TCDD	DMBA	Control	DMBA
Stimulated
Cyp1b1	3.5	2.5	n/a	n/a	A
Cyp1a1	9.7	15.0	nd
Ahrr	3.8	4.1	nd
IL6	3.0	ns	2.5	4.5	B
Tnfa	2.0	ns	1.5	3.0
	2.0	ns	1.5	4.0
	2.9	ns	2.5	4.4
	2.4	1.3	2.2	ns	C
Fau	2.0	1.5	2.2	1.3
	1.7	ns	1.6	ns
Creb3l3	2.9	ns	7.6	1.9
	2.6	1.3	2.7	ns
	1.5	ns	5.0	2.2
Polr2a	5.0	ns	6.2	2.3
Klf4	3.6	1.3	2.1	1.7	D
Nr4a1	3.6	ns	2.0	2.2
	8.5	ns	2.6	3.0
	1.8	1.4	2.8	2.4
Socs3	2.0	1.5	2.0	2.8
Rag1	−1.8	ns		−3.0	E
Erdr1	2.5	ns	−2.4	−2.4
Col5a3	−1.5	ns		−2.0
Faim3	−1.5	1.3	−2.9	−1.5
Spp1	−3.0	1.6	−2.2	−1.9

Fold-changes (FC) are all significant; .
ns: not significant.
n/a: not available.
nd: not detectable.
: relative to Cyp1b1-ko control genotype effect on PAH.
responses from family members (a1, Egr1; a2, Hbb-a1/a2; a3, Hspa-lb; a4, Cxcl9; a5, Hoxb9; a6, Sox15; a7, Gimap1).
response from family member, Alas1.
significant difference from BP.