Innervation of Cochlear Hair Cells by Human Induced Pluripotent Stem Cell-Derived Neurons In Vitro

Figure 1

Timeline for coculture experiments. The stem cell lines were differentiated towards a neuronal lineage for either 21 or 28 days in vitro (DIV). For the cochlear explant cocultures, 21 DIV neurospheres were cocultured with cochlear explants for up to 10 days (red). For the synapse forming assay experiments, 21 (red) and 28 (blue) DIV neurospheres were cocultured with hair cells for 10 days in each case. The cocultures were treated with NBM, BDNF, and NT-3. DIV: days in vitro; NBM: neurobasal media; EGF: epidermal growth factor; bFGF: basic fibroblast growth factor; HFF: human foreskin fibroblasts; CE: cochlear explant; HC: hair cell; NS: neurosphere; and Y27: small molecule Y27632.