Maintenance of Self-Renewal and Pluripotency in J1 Mouse Embryonic Stem Cells through Regulating Transcription Factor and MicroRNA Expression Induced by PD0325901

Figure 3

Experimental scheme for sample preparation of small RNA deep-sequencing and microarray analysis. (a) Experimental scheme for small RNA deep-sequencing and microarray analysis. J1 mESCs cultured in LIF containing media were treated with 1 μM PD or equal volume of DMSO (control) for 24 h, and then the total RNAs were extracted and qualified RNAs were analyzed by microarray gene expression profiling and small RNA deep-sequencing to identify differentially expressed mRNAs and miRNAs. (b) Comparison of the known miRNA expression between DMSO- and PD-treated samples. The scatter plots show the distribution of the detected miRNAs with or without 1 μM PD treatment in mESC medium for 24 h. Significant regulated miRNAs with 1.5-fold change are marked red (upregulated) and green (downregulated).

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