Maintenance of Self-Renewal and Pluripotency in J1 Mouse Embryonic Stem Cells through Regulating Transcription Factor and MicroRNA Expression Induced by PD0325901
PD regulate the expression of the ESCC family of miRNAs in mESCs. (a–e) Relative fold change of mature ESCC family of miRNAs. J1 mESCs were treated with 1 μM PD or equal volume of DMSO (control) for 24 h, and then the total RNAs were extracted and qualified RNAs were analyzed by small RNA deep-sequencing to identify differentially expressed miRNA. miR-302-367 cluster, miR-290-295 cluster, miR-17-92b cluster, miR-106a-363 cluster, and miR-106b-25 cluster in control and PD-treated J1 mESCs detected by small RNA deep-sequencing. (f) RT-qPCR validation of differentially expressed miRNA in PD-treated J1 mESCs. J1 mESCs were treated with 1 μM PD for 24 h, and then the expression of miRNAs was determined by RT-qPCR. Error bars indicate mean ± SD of three independent experiments, compared with controls.
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