Maintenance of Self-Renewal and Pluripotency in J1 Mouse Embryonic Stem Cells through Regulating Transcription Factor and MicroRNA Expression Induced by PD0325901

Figure 4

PD regulate the expression of the ESCC family of miRNAs in mESCs. (a–e) Relative fold change of mature ESCC family of miRNAs. J1 mESCs were treated with 1 μM PD or equal volume of DMSO (control) for 24 h, and then the total RNAs were extracted and qualified RNAs were analyzed by small RNA deep-sequencing to identify differentially expressed miRNA. miR-302-367 cluster, miR-290-295 cluster, miR-17-92b cluster, miR-106a-363 cluster, and miR-106b-25 cluster in control and PD-treated J1 mESCs detected by small RNA deep-sequencing. (f) RT-qPCR validation of differentially expressed miRNA in PD-treated J1 mESCs. J1 mESCs were treated with 1 μM PD for 24 h, and then the expression of miRNAs was determined by RT-qPCR. Error bars indicate mean ± SD of three independent experiments, compared with controls.

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