Effects on Proliferation and Differentiation of Human Umbilical Cord-Derived Mesenchymal Stem Cells Engineered to Express Neurotrophic Factors

Figure 2

HUMSCs were transduced with control lentiviral vectors or those designed to overexpress NTFs. Overexpression of NTFs was then confirmed at both mRNA and protein levels. (a) mRNA was extracted from HUMSCs 3 days after transduction and measured by real-time reverse transcription polymerase chain reaction (). (b) Protein levels of NTF were measured in supernatant of HUMSCs using enzyme-linked immunosorbent assay (). (c) Protein levels of NTF measured in HUMSCs using western blot. (d) Semiquantitative analysis of results of western blot. versus control ().

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