Exosomes from Human Umbilical Cord Mesenchymal Stem Cells: Identification, Purification, and Biological Characteristics

Figure 3

HucMSCs-exosomes protect cell viability in vitro. (a) MTT assay. H9C2(2-1), HL-7702, and NRK-52E cells were pretreated with different sources of hucMSCs-exosomes (800 μg/mL) for 24 hours, followed by exposure to H₂O₂ (300 μM) for 24 hours. Results were shown as mean ± SD (). and compared to H₂O₂ group. (b) MTT assay. H9C2(2-1), HL-7702, and NRK-52E cells were pretreated with different sources of hucMSCs-exosomes (800 μg/mL) or exosomes-free conditioned medium collected from hucMSCs (EX-free-CM) for 24 hours, followed by exposure to H₂O₂ (300 μM) for 24 hours. Results were shown as mean ± SD (). and compared to H₂O₂ group. (c) Western blotting analysis of phosphorylated p38. H9C2(2-1), HL-7702, and NRK-52E cells were pretreated with hucMSCs-exosomes (800 μg/mL) followed by exposure to H₂O₂ as described above.

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