Exosomes from Human Umbilical Cord Mesenchymal Stem Cells: Identification, Purification, and Biological Characteristics

Figure 5

HucMSCs-exosomes inhibit H₂O₂-induced apoptosis. (a) TUNEL staining. H9C2(2-1), HL-7702, and NRK-52E cells were pretreated with hucMSCs-exosomes (800 μg/mL) for 24 hours, followed by exposure to H₂O₂ (300, 300, and 500 μM) for different time points (24, 6, and 6 hours). (A–C) H9C2(2-1); (D–F) HL-7702; and (G–I) NRK-52E. Control (A, D, and G); H₂O₂ (B, E, and H); hucMSCs-exosomes (C, F, and I). Scale bar: 100 μm. (b) Quantitative analysis of the percentage of TUNEL-positive cells. compared to H₂O₂ group.

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