Enhanced Osteogenic and Vasculogenic Differentiation Potential of Human Adipose Stem Cells on Biphasic Calcium Phosphate Scaffolds in Fibrin Gels
Gene expression of the proliferation marker KI67 and osteogenic differentiation markers RUNX2, ON, DMP1, COL1, and COL3 in BCP60/40- and BCP20/80-based composites and fibrin gels at days 1, 7, and 11 of culture. (a) BCP60/40- and BCP20/80-based composites showed increased KI67 gene expression compared to fibrin gels at day 11. (b) RUNX2 gene expression was similar for BCP60/40- and BCP20/80-based composites and fibrin gels at all time points. (c) BCP60/40- and BCP20/80-based composites showed similar ON gene expression at all time points. (d) BCP20/80-based composites showed increased DMP1 gene expression compared to BCP60/40-based composites, as well as to fibrin gels at days 1 and 11. (e, f) Gene expression of COL1 and COL3 in BCP60/40- and BCP20/80-based composites was similar at all time points. Values are mean ± SEM (–12).Significant effect of BCP60/40- or BCP20/80-based composites compared to fibrin gels, .Significantly different from BCP60/40-based composites, . BCP, biphasic calcium phosphate; KI67, proliferation marker; RUNX2, runt-related transcription factor-2; ON, osteonectin; DMP1, dentin matrix acidic phosphoprotein-1; COL1, collagen-1; COL3, collagen-3.
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