Enhanced Osteogenic and Vasculogenic Differentiation Potential of Human Adipose Stem Cells on Biphasic Calcium Phosphate Scaffolds in Fibrin Gels

Figure 3

Gene expression of the proliferation marker KI67 and osteogenic differentiation markers RUNX2, ON, DMP1, COL1, and COL3 in BCP60/40- and BCP20/80-based composites and fibrin gels at days 1, 7, and 11 of culture. (a) BCP60/40- and BCP20/80-based composites showed increased KI67 gene expression compared to fibrin gels at day 11. (b) RUNX2 gene expression was similar for BCP60/40- and BCP20/80-based composites and fibrin gels at all time points. (c) BCP60/40- and BCP20/80-based composites showed similar ON gene expression at all time points. (d) BCP20/80-based composites showed increased DMP1 gene expression compared to BCP60/40-based composites, as well as to fibrin gels at days 1 and 11. (e, f) Gene expression of COL1 and COL3 in BCP60/40- and BCP20/80-based composites was similar at all time points. Values are mean ± SEM (–12).Significant effect of BCP60/40- or BCP20/80-based composites compared to fibrin gels, .Significantly different from BCP60/40-based composites, . BCP, biphasic calcium phosphate; KI67, proliferation marker; RUNX2, runt-related transcription factor-2; ON, osteonectin; DMP1, dentin matrix acidic phosphoprotein-1; COL1, collagen-1; COL3, collagen-3.

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