Investigation of the Cell Surface Proteome of Human Periodontal Ligament Stem Cells

Figure 1

Representative raw 2DE gel of CyDye labelled proteins of ex vivo expanded human periodontal ligament stem cells and the location of proteins identified on the raw 2DE image. Following cell surface labelling with CyDye and membrane protein enrichment, proteins were separated by 2DE using a pI range of pH 3–10 and a molecular weight range of 10–110 kDa. Following image analysis, 80 well-resolved protein spots were detected. A total of 32 membrane-associated protein spots were consistently found on replicate gels.