Senescence-Associated Molecular and Epigenetic Alterations in Mesenchymal Stem Cell Cultures from Amniotic Fluid of Normal and Fetus-Affected Pregnancy

Figure 2

Immunophenotype and stemness markers of MSCs derived from AF of normal and pathological gestation during cultivation. (a) Representative flow cytometry histograms of cell surface antigen markers of MSCs obtained from AF of normal gestation (N) at passage 3 and the late passage 8. Cells were positive for staining with CD44, CD90, and CD105 antibodies and negative for CD34 antibody. The appropriate IgG isotype was used as a control. (b) The percentage mean of CD44, CD90, and CD105 positive cells in MSCs cultures derived from AF of pathological gestation (P) at passages 3 and 8. (c) The expression of Nanog, Oct4, Sox2, and Rex1 from AF samples of normal (N) and pathological gestation (P) at passages 3, 6, and 8 was analyzed by RT-qPCR and normalized to GAPDH expression levels. Results are presented as the mean ± SD (, from each group). and were considered as significant changes.

(a)

(b)

(c)