Senescence-Associated Molecular and Epigenetic Alterations in Mesenchymal Stem Cell Cultures from Amniotic Fluid of Normal and Fetus-Affected Pregnancy

Figure 3

Characterization of senescence-associated markers in MSCs cultures during cultivation. (a) Differential proliferation of MSCs in two groups of cell cultures (I and II) during cultivation to passages 3, 5, and 8 (, for each group). (b) Nonstained (NS) and SA-β-gal positive cells at passages 6 and 9 in two groups of MSCs cultures. (c) Differential mRNA expression of senescence-associated markers in MSCs cultures (I gr and II gr) at passage 3 and the late passage was determined by RT-qPCR. Normalization to GAPDH and fold expression difference compared with passage 3 calculated using a comparative threshold cycle delta-delta Ct method. The data is presented as the mean ± SD (, from each group). , , and were considered as significant changes.

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