Senescence-Associated Molecular and Epigenetic Alterations in Mesenchymal Stem Cell Cultures from Amniotic Fluid of Normal and Fetus-Affected Pregnancy

Figure 4

Differential expression of miRNAs and stem cell markers in MSCs cultures during passaging. RT-qPCR analysis of miR-17 and miR-21 (a) and Nanog, Oct4, Sox2, and Rex1 (b) from cell cultures of two groups (I and II) at passage 3 and the late passage. Normalization to calibrator sample (a) or GAPDH (b) and fold expression difference as compared with passage 3 calculated using a comparative threshold cycle delta-delta Ct method. The data is presented as the mean ± SD (, from each group). and were considered as significant changes.

(a)

(b)