Mesenchymal Stromal Cells Derived Extracellular Vesicles Ameliorate Acute Renal Ischemia Reperfusion Injury by Inhibition of Mitochondrial Fission through miR-30

Figure 1

hWJMSC-EVs alleviate mitochondrial fragmentation and DRP1 activation in the model of IRI in vitro and in vivo. (a) Two hours after the treatment with azide, the renal tubular epithelial cells were stained with MitoTracker Red. The morphological alterations of mitochondria in renal tubular epithelial cells were imaged using a laser scanning confocal microscope (the original magnification is 630x). Bar = 5 um. (b) The percentage of cells undergoing mitochondrial fission. (c, d) Mitochondrial DRP1 expression in kidney tissues was measured by western blot analysis at 24 hours after sham, IRI, or EVs treatment. VDAC antibody served as a loading control. Data in (b) and (d) are presented as mean ± SD; ; , versus sham; , versus vehicle.

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