Mesenchymal Stem Cells Derived from Human Exocrine Pancreas Spontaneously Express Pancreas Progenitor-Cell Markers in a Cell-Passage-Dependent Manner

Table 1

Primers used for PCR amplification.

Gene		Sequence ()	(°C)	Product size (bp)
Insulin	Forward	CATGGCCCTGTGGATGCGC	64	331
	Reverse	AGTTGCAGTAGTTCTCCAG	52.5
Glucagon	Forward	CCCAAGATTTTGTGCAGTGGTT	57.5	221
	Reverse	GCGGCCAAGTTCTTCAACAAT	57.7
Amylase	Forward	GGTTCAGGTCTCTCCACCAA	53.5	214
	Reverse	TCCTGCACTCACAGCGTTAC	53.4
Cytokeratin 19	Forward	AGGTGGATTCCGCTCCGGGCA	68.2	460
	Reverse	ATCTTCCTGTCCCTCGAGCA	55.8
Pdx1	Forward	GCCCCCGCCGCCGCCGCCGCA	75.1	231
	Reverse	GGCAGCTGGACGCGGTTGGGC	71.2
Ngn3	Forward	GTAGAAAGGATGACGCCTCAACC	57.5	241
	Reverse	TCAGTGCCAACTCGCTCTTAGG	58.1
MafA	Forward	TTCAGCAAGGAGGAGGTCAT	53.3	216
	Reverse	CGCCAGCTTCTCGTATTTCT	53.6
Nkx2.2	Forward	TTCTACGACAGCAGCGACAACC	58.9	393
	Reverse	CGTCACCTCCATACCTTTCTCG	57.2
Nkx6.1	Forward	ACACGAGACCCACTTTTTCCG	57.6	336
	Reverse	TGCTGGACTTGTGCTTCTTCAAC	58
Isl1	Forward	ATTTCCCTATGTGTTGGTTGCG	57.4	229
	Reverse	CGTTCTTGCTGAAGCCGATG	58
Pax4	Forward	TTTGTGCTGAAGGGCTTTGC	57.5	216
	Reverse	GGGAGAAGATAGTCCGATTCCG	57.6
Pax6	Forward	CGGCAGAAGATTGTAGAG	44.8	290
	Reverse	GATGACACGCTTGGTATG	45.8
Sox9	Forward	TACGACTACACCGACCACCA	53.4	213
	Reverse	TCAAGGTCGAGTGAGCTGTG	53.3
P48	Forward	CTGGAATGCCTGGACTCAAT	53.6	232
	Reverse	AGGTTGCTTTTCCTTCAGCA	53.8
CD44	Forward	AAGGTGGAGCAAACACAACC	53.6	151
	Reverse	AGCTTTTTCTTCTGCCCACA	53.8
CD90	Forward	CTAGTGGACCAGAGCCTTCG	53.7	236
	Reverse	TGGAGTGCACACGTGTAGGT	53.4
Vimentin	Forward	GAGAACTTTGCCGTTGAAGC	53.8	170
	Reverse	TCCAGCAGCTTCCTGTAGGT	53.6
Desmin	Forward	AAGCTGCAGGAGGAGATTCA	53.7	240
	Reverse	GGCAGTGAGGTCTGGCTTAG	53.6
Beta actin	Forward	AGAGCTACGAGCTGCCTGAC	53.5	184
	Reverse	AGCACTGTGTTGGCGTACAG	53.4