The Influence of Aging on the Regenerative Potential of Human Adipose Derived Mesenchymal Stem Cells

Figure 4

Characterization of age-dependent apoptosis. (a) Cells stained for senescence showing cells with β-galactosidase accumulation (A–D), Calcein-AM (E–H), and propidium iodide (I–L) for live-dead cells identification. (b) β-galactosidase absorbance measurement. (c) Percentage of apoptotic cells calculated from Calcein-AM/propidium iodide staining, (d) evaluation of p53, and (e) p21 on the mRNA level. Results expressed as mean ± SD. value <0.05.

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