Mesenchymal Stem Cells Loaded with p5, Derived from CDK5 Activator p35, Inhibit Calcium-Induced CDK5 Activation in Endothelial Cells

Figure 5

p5 and conditioned medium (CM) from p5-primed hADMSCs protected BAECs from calpain-induced cytotoxicity. The cultured BAECs were treated with p5 or CM from 12 μg/mL p5-primed hADMSCs collected on Day 1 for 1 hour and then further treated with 5 μM calcium ionophore A23187 and 2.5 mM CaCl₂ for 10 minutes (a) or 2 hours (b). The cell proliferation was monitored for 3 consecutive days using the alamarBlue assay. Data are means ± SD (error bars). Significance (denoted as ) was calculated compared to the control using Student’s -test (with < 0.05, = 3).

(a)

(b)