High Content Analysis of Human Pluripotent Stem Cell Derived Hepatocytes Reveals Drug Induced Steatosis and Phospholipidosis

Table 2

IC₅₀/EC₅₀ values for drug induced effects on different parameters measured in hiPSC derived hepatocytes (hiPS-HEP) and HepG2.

Compound	Amiodarone		Doxycycline		Tetracycline
Cell line	hiPS-HEP	HepG2	hiPS-HEP	HepG2	hiPS-HEP	HepG2
Fixed
Nucleus
Cell count	31.0	7.6	107	165	432	558
MI	95.7	140	47.2	892	211	348
Area	2.2	39.5	96.1	OR	105	464
Steatosis
Count	21.0	19.5	115	260	184	36.3
MI	34.1	23.6	441	OR	418	361
Area	25.5	30.6	—	398	213	287
Phospholipidosis
Count	6.1	8.9	NA	NA	NA	NA
MI	6.0	35.6	NA	NA	NA	NA
Area	4.5	7.3	NA	NA	NA	NA
Live
Nucleus
Cell count	21.9	7.2	69.4	120.3	243	836
MI	22.4	25.0	120	153	380	959
Area	10.4	29.1	204.3	—	211	336
MMP
MI	20.8	345	644	622	—	265
ROS
MI	26.5	4.6	10.5	115	160	1149
PMP
MI	42.2	147	26.9	OR	OR	OR

Four-parameter variable slope curve fit using the least squares method was applied for generating dose response curves in GraphPad Prism software. Parameters with the lowest and second lowest IC₅₀/EC₅₀ values are denoted in bold; values in italics had an ambiguous near complete dose response curve fit; [OR], curves which were out of range. Values denoted are mean values from three different experimental batches (). All values are represented as μM.