Research Article

Long-Term Oocyte-Like Cell Development in Cultures Derived from Neonatal Marmoset Monkey Ovary

Figure 3

RT-qPCR analysis of OCCs in different passages. Real-time quantitative RT-PCR analysis of selected key pluripotency and germ cell markers in OCCs. OCT4, NANOG, SALL4, and LIN28 are all robustly expressed in pluripotent stem cells as well as in primitive germ cells. In contrast OCT4 and NANOG are absent from OCCs. SALL4 and LIN28 were absent or very low at low passages and increased in expression at higher passages. VASA is a general germ cell marker and was detected in later OCC passages and in the neonatal ovary.
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