Phenotypic, Functional, and Safety Control at Preimplantation Phase of MSC-Based Therapy

Figure 3

(a) Multilineage mesodermal differentiation potential of WJ-MSC. Positive results of differentiation were noticed exclusively in WJ-MSC(M) with only exception of slightly positive chondrogenesis observed in WJ-MSC(E) cultures. (b) Neural/neuronal differentiation of WJ-MSC. Expression of neural/neuronal markers was found on higher levels in mechanically than enzymatically isolated cells. Scale bars: 100 μm. (c) α-SMA expression analysis. In mechanically isolated MSC a higher expression of primitive cell marker α-SMA has been observed. The results present mean values of 3 isolations ± SD, . (d) Quantitative neural/neuronal markers mRNA analysis. Relative genes expression of WJ-MSC(M) at 2nd-3rd passages normalized to the reference gene ACTB was compared to WJ-MSC(E) as calibrator group. The results are presented as mean values of 3 isolations ± SD; ; ; .

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