Resveratrol Enhances Cardiomyocyte Differentiation of Human Induced Pluripotent Stem Cells through Inhibiting Canonical WNT Signal Pathway and Enhancing Serum Response Factor-miR-1 Axis

Figure 1

RSV has no effect on the self-renewal of human iPSCs but can promote mesoderm differentiation. (a) Representative images of the human iPSCs morphology 5 days after different concentrations of resveratrol treatment (10x). (b) Quantitative RT-PCR (qRT-PCR) analysis of pluripotent markers Oct4, Sox2, NANOG, and KLF4 in human iPSCs 5 days after RSV administration. (c) Cell proliferation assay was performed using CCK-8. (d–g) Under embryoid body (EB) condition, dynamic gene expressing profile of pluripotency (d), endoderm (e), mesoderm (f), and ectoderm markers (g) were detected by qRT-PCR analysis. Data represent the mean ± s.d. of three biological replicates. .

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