Resveratrol Enhances Cardiomyocyte Differentiation of Human Induced Pluripotent Stem Cells through Inhibiting Canonical WNT Signal Pathway and Enhancing Serum Response Factor-miR-1 Axis

Figure 2

RSV enhances CM differentiation of human iPSCs. (a) A protocol outlined describing the cardiovascular differentiation strategy. (b) Ratios of beating EBs in control and RSV group from day 8 to day 24 of differentiation, respectively (). (c) Gene expression profile of pluripotency, cardiomyocyte specific markers during differentiation process by qRT-PCR analysis. (d) A representative immunofluorescent image of comparison of cTnT positive CMs from dissociated day 24 EBs between control and RSV groups (40x). (e) Flow cytometry was performed to compare the percentage of cTnT positive cells between control and RSV group at day 24. (f) cTnT protein level was further compared using Western Blot method at day 24. (g) Western Blot analysis was further performed to confirm the cardiomyocyte specific genes (as shown in (c)) at protein levels. Data represent the mean ± s.d. of three biological replicates. , .

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