Resveratrol Enhances Cardiomyocyte Differentiation of Human Induced Pluripotent Stem Cells through Inhibiting Canonical WNT Signal Pathway and Enhancing Serum Response Factor-miR-1 Axis

Figure 3

RSV enhances CM differentiation partially through inhibition of WNT signal pathway. (a) An outline describing the administrations of pathway modulators during CM differentiation. (b) TOP Flash assay of human iPSCs treated with different concentrations of RSV in suspension culture condition at day 4. (c) From day 0 to day 5, TOP Flash assays were performed, respectively, after 50 μM RSV incubation. (d, e). The effects of different concentrations of DKK1, WNT inhibitor (d) and WNT3A, WNT activator (e) on the ratio of beating EBs were evaluated on day 24 (). Data represent the mean ± s.d. of three biological replicates. compared with control; compared with RSV alone.

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