The New Role of CD163 in the Differentiation of Bone Marrow Stromal Cells into Vascular Endothelial-Like Cells

Figure 4

CD163 acted downstream Hmbox1 in differentiation of BMSCs towards a VEC lineage. (a) Western blot analysis of the protein level of Hmbox1 after overexpression or knockdown of CD163. (b) Relative protein levels of Hmbox1 were calculated based on densitometry analysis. (c) Western blot analysis of the protein level of Hmbox1 and CD163 after overexpression or knockdown of Hmbox1. For Hmbox1 overexpression, BMSCs were transfected with pCMV6 empty vector (as control) at 3.2 μg/mL or pCMV6-Hmbox1 at 0.8, 1.6, or 3.2 μg/mL for 4 h and cultured for 48 h. For Hmbox1 interference, cells were subjected with 80 nmol/L scrambled siRNA or 20, 40, and 80 nmol/L Hmbox1 siRNA for 6 h and cultured for 48 h. Scr: scrambled RNA as negative control. ((d)-(e)) Relative protein levels of Hmbox1 and CD163 were calculated based on densitometry analysis. Data are mean ± SEM. , versus control (Ctr), .

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