Semaphorin 3A Shifts Adipose Mesenchymal Stem Cells towards Osteogenic Phenotype and Promotes Bone Regeneration In Vivo

Figure 4

Sema3A infected ASCs decrease the immune cell infiltration while increasing fibroblast-like cells migrated to the implants. (a) The scaffolds were harvested 5 days after transplantation and immune-stained with collagen. (A) No GFP⁺ fibroblast-like cells were found in the empty implants and multiple inflammatory cells were shown, (B) a small fraction of fibroblast-like cells were found in implants seeded with , and (C) Multiple fibroblast-like cells were found migrated and migrating towards the scaffold in implants seeded with . (b) Quantification data of survival GFP⁺ ASCs in the scaffold. (c) Quantification data of migrated fibroblast-like cells. Mean ± SD, , and .

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