Human Adipose-Derived Mesenchymal Stem Cells Cryopreservation and Thawing Decrease α4-Integrin Expression

Table 1

Panel of monoclonal antibodies used in this study for immunophenotypic characterization.

Marker	Clone	Reactivity	Fluorochrome	Function
CD34	581/CD34	Human	FITC	Stem cell marker (precursor), also found in hematopoietic progenitor cells, vascular endothelium, and fibroblasts of the same tissue. Probably, it works as a transduction signer and has a function in endothelium specific antigen adhesion.
CD45 (common leukocyte antigen)	HI30	Human	PE	Leukocytes marker. CD45 proteins are located in all hematopoietic cells, but not in erythrocytes.
CD49d (integrin α4)	9F10	Human	PE	Transmembrane glycoprotein, integrin α4. It makes several cell-cell interaction and cell-matrix and participated in cellular adhesion.
CD73 (ecto-5′-nucleotidase)	AD2	Human	PE	It have been suggested that this marker can mediate costimulator signals in the cells T activation and endothelium as well catalyses the dephosphorylation of adenosine monophosphate in adenosine.
CD90 (THy-1)	5E10	Human	FITC	The role of interaction in cell-cell and cell-matrix has been speculated, with related neurites growth, nerve regeneration, apoptosis, metaphase, inflammation, and fibrosis.
CD105 (endoglin)	266	Human	Purified	Responsiveness modulator of TGF-β cellular complex.