Extracellular Recordings of Patterned Human Pluripotent Stem Cell-Derived Cardiomyocytes on Aligned Fibers
IMR hiPS-cardiomyocytes (CMs) on PMGI fibers. (a–d) Fluorescence image of CMs cultured for 14 days on aligned fibers (AFs) and gelatin-coated flat substrates (Flat). (a, b) α-actinin is labeled in red and (c-d) TnT2 in green. The color on fiber is due to autofluorescence of PMGI. The scale bar in (a, b) = 50 μm. The scale bar in (c, d) = 200 μm. (e) Transmission electron microcopy images of CMs cultured for 14 days on AFs. The red arrows mark the fibers. The scale bar = 5 μm. (f, g) Higher magnitude TEM images of CMs cultured for 14 days on AFs (f) and Flat (g). The red and blue arrows mark the fibers and the sarcomeric bundles, respectively. The scale bar = 500 nm.