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Stem Cells International
Volume 2016, Article ID 2650849, 9 pages
http://dx.doi.org/10.1155/2016/2650849
Research Article

Effect of Periodic Granulocyte Colony-Stimulating Factor Administration on Endothelial Progenitor Cells and Different Monocyte Subsets in Pediatric Patients with Muscular Dystrophies

1Department of Regenerative Medicine and Immune Regulation, Medical University of Bialystok, 15-269 Bialystok, Poland
2Department of Pediatric Rehabilitation and Center of Early Support for Handicapped Children “Give a Chance”, Medical University of Bialystok, 15-274 Bialystok, Poland
3Department of Immunology, Medical University of Bialystok, 15-269 Bialystok, Poland
4Department of Allergology and Internal Medicine, Medical University of Bialystok, 15-276 Bialystok, Poland

Received 5 May 2015; Accepted 26 July 2015

Academic Editor: Tao Wang

Copyright © 2016 Andrzej Eljaszewicz et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Muscular dystrophies (MD) are heterogeneous group of diseases characterized by progressive muscle dysfunction. There is a large body of evidence indicating that angiogenesis is impaired in muscles of MD patients. Therefore, induction of dystrophic muscle revascularization should become a novel approach aimed at diminishing the extent of myocyte damage. Recently, we and others demonstrated that administration of granulocyte colony-stimulating factor (G-CSF) resulted in clinical improvement of patients with neuromuscular disorders. To date, however, the exact mechanisms underlying these beneficial effects of G-CSF have not been fully understood. Here we used flow cytometry to quantitate numbers of CD34+ cells, endothelial progenitor cells, and different monocyte subsets in peripheral blood of pediatric MD patients treated with repetitive courses of G-CSF administration. We showed that repetitive cycles of G-CSF administration induced efficient mobilization of above-mentioned cells including cells with proangiogenic potential. These findings contribute to better understanding the beneficial clinical effects of G-CSF in pediatric MD patients.