Improvement in Isolation and Identification of Mouse Oogonial Stem Cells

Figure 3

PCR for detection of EGFP gene integrated into the genomes of fetus and offspring. (a–c) Examples of PCR analysis of fetus, offspring and the wild types for detection of EGFP integrated into recipient genomes. There were positive outcomes of 366 bp PCR product in both fetus (a) and offspring (b), but no positive band was found in the wild types (c) which served as the negative control. (d) Southern blot analysis of tail DNA from six offspring, produced from the same recipient mouse and one wild type using a 523-bp PCR product from the control plasmid with primers for EGFP gene as a probe, showing five positive and one negative. Genomic DNA was digested with PstI. Size markers are indicated in the right of southern blot. Lanes 1–6, transgenic mice; lane 7, WT mouse; and lane 8, the control plasmid.

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