Research Article
Improvement in Isolation and Identification of Mouse Oogonial Stem Cells
Table 1
Details regarding PCR primers used in RT-PCR for mouse ovary and OSCs.
| | Gene | Accession number | Product size (bp) | Primer sequence (5′-3′) |
| | Gdf9 | NM_008110 | 709 | F: TGCCTCCTTCCCTCATCTTG
R: CACTTCCCCCGCTCACACAG |
| | Nobox | NM_130869 | 379 | F: CCCTTCAGTCACAGTTTCCGT
R: GTCTCTACTCTAGTGCCTTCG |
| | Zp3 | NM_011776 | 183 | F: CCGAGCTGTGCAATTCCCAGA
R: AACCCTCTGAGCCAAGGGTGA |
| | Fragilis | NM_012013 | 151 | F: GTTATCACCATTGTTAGTGTCATC
R: AATGAGTGTTACACCTGCGTG |
| | MVH | NM_010029 | 216 | F: ACCCAGTTTGGTCATTCAGTTCG
R: TTGTTCCTTTGATGGCATTCCTG |
| | Prdm1 | NM_007548 | 149 | F: ACAGAATGGCAAGATCAAGTATGA
R: GGTGGGCGAGCTGAGTAAAA |
| | Tert | NM_009354 | 120 | F: GCTTCCCTTTGACCAGCGTGTTA
R: GCCTTTAGTGTCATTCCTGGATTCTT |
| | Dazl | NM_010021 | 358 | F: GTTAGGATGGATGAAACCGAAAT
R: ATGCCTGAACATACTGAGTGATA |
| | Gapdh | NM_008084 | 458 | F: GTCCCGTAGACAAAATGGTGA
R: TGCATTGCTGACAATCTTGAG |
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