Differential Proteomic Analysis of Human Placenta-Derived Mesenchymal Stem Cells Cultured on Normal Tissue Culture Surface and Hyaluronan-Coated Surface

Figure 1

Hyaluronan-coated surface affected cell proliferation of PDMSC. (a) The PDMSC grown on tissue-culture polystyrene surface differentiated to adipocytes, chondrocytes, and osteocytes after induction for 4 weeks. Scale bar = 100 μm. (b) The PDMSC presented fibroblastic morphology on tissue-culture polystyrene surface (TCS) and formed aggregate on tissue-culture polystyrene surface coated with 30 μg/mL hyaluronan (CHA). The cells at P5 are noted as young and P17 as old. In CHA, the culture of PDMSC on HA-coated surface began at P2; thus, P2 + 3 passages (P5) and P2 + 15 passages (P17) for young and old CHA, respectively. Scale bar = 100 μm. (c) The proliferation of PDMSC on TCS and CHA grown for a period of 5 weeks. Each of the data points represents cumulated population doubling for one passage. Three independent experiments were performed in TCS and CHA and data are represented in mean ± SD. value .

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