Research Article
Differential Proteomic Analysis of Human Placenta-Derived Mesenchymal Stem Cells Cultured on Normal Tissue Culture Surface and Hyaluronan-Coated Surface
Figure 2
Proteomic workflow and incorporation rate of SILAC-labeled PDMSC. (a) The protein lysates collected at P5 and P17 for PDMSC cultured on TCS and CHA were separated on 8% SDS gel and stained with coomassie blue. The amounts of protein loaded were 10 μg (Lane A) and 30 μg (Lane B), respectively, for YTCS, YCHA, OTCS, and OCHA. The symbol (#) represents varied protein bands in the OCHA (Lane B) compared to OTCS (Lane B). (b) The illustration shows experimental workflow. (c-d) Heavy amino acids incorporation for OTCSH after 5-6 doublings. (e-f) Heavy amino acids incorporation for YCHAH after 5-6 doublings. Incorporation efficiency of the heavy arginine and lysine for PDMSC were determined before further protein identification and quantitation analysis using LC-MS/MS.
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