Differential Proteomic Analysis of Human Placenta-Derived Mesenchymal Stem Cells Cultured on Normal Tissue Culture Surface and Hyaluronan-Coated Surface

Figure 3

The aging phenomena in HA absence detected high percentages of signal transduction and structural proteins. Pie charts representing the total proteins identified with ratio above 1.5 in the (a) OTCS^H versus YTCS^L, (b) OCHA^L versus OTCS^H, and (c) OCHA^L versus YCHA^H groups, respectively. (a1) The OTCS^H had 0% of replication-related proteins, showing that replication had slowed down or ceased. (a2) The upregulated proteins in OTCS^H had 30.9% of structural proteins and 3.8% of downregulated proteins cell structural. Cell structure may have played a major role in the aging cells. (b1) In comparison to OTCS^H, OCHA^L had 10.4% upregulated proteins of biosynthesis/degradation proteins, which correlated with the high percentage of metabolic proteins (20.8%), indicating that OCHA^L had higher metabolic activity. (b2) The group OCHA^L had 43.5% downregulated proteins for signal transduction. (c1) The metabolic activity (28.1%) in OCHA^L was higher compared to YCHA^H, which may have correlated with the protein biosynthesis/degradation activity of 18.8%. The aging phenomena in the OCHA^H differed from that in OTCS^H in (a1), where the upregulated 12.7% of metabolic and 1.8% biosynthesis/degradation proteins. (c2) Epigenetics consisted 18.8% of the downregulated proteins in OCHA^L, suggesting epigenetic regulation of cells after long-term HA presence.