The Regulatory Effects of Long Noncoding RNA-ANCR on Dental Tissue-Derived Stem Cells

Figure 5

The effect of ANCR-RNAi on neurogenic differentiation in DTSCs. Immunofluorescence results showed that the expression of βIII-TUBULIN on DPSC/ANCR-RNAi (a), PDLSC/ANCR-RNAi (b), and SCAP/ANCR-RNAi (c) cells was stronger than control groups. The green is the color of GFP (Green Fluorescent Protein) transfected by the vector. The red is the color of βIII-TUBULIN stained by DyLight 405. The morphology of DPSC/ANCR-RNAi and PDLSC/ANCR-RNAi cells was remarkably changed. The mRNA expressions of βIII-TUBULIN, GAP43, NEFL, and NESTIN were upregulated in DPSC/ANCR-RNAi cells (d) after 2 weeks of induction. In PDLSC/ANCR-RNAi cells (e), βIII-TUBULIN and NESTIN were upregulated, while GAP43 and NEFL had little change compared with control groups. In SCAP/ANCR-RNAi cells (f), βIII-TUBULIN, NEFL, and NESTIN were upregulated while GAP43 had little change compared with control groups. when compared with the control group.