BMSCs Interactions with Adventitial Fibroblasts Display Smooth Muscle Cell Lineage Potential in Differentiation and Migration That Contributes to Neointimal Formation

Figure 1

Characteristics of cultured vascular adventitial fibroblasts and expression of α-SMA, TGF-β1, MMP-1, and NF-κB. (a) Morphological observation and representative photographs of fibroblasts. (A) Fibroblasts grown from tissue pieces of vascular adventitia (×100). (B) The 2-3 generations of fibroblasts showed a robust growth with a multilayer overlapping crest shape (×100). (C) Fibroblasts stained with Cy3 antibody (red) indicating intracellular α-SMA after TGF-β1 treatment (×400). (b) Expressions of α-SMA, TGF-β1, MMP-1, and NF-κB mRNA in adventitial fibroblasts in the model groups or the control group with or without TGF-β1. (c) Significant differences among groups were indicated by RT-PCR. Averages of at least three independent experiments (). Bars indicated the mean ± SD. compared to the control (model versus control, ; the model group for 3 or 7 days versus 1 day, ).

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