BMSCs Interactions with Adventitial Fibroblasts Display Smooth Muscle Cell Lineage Potential in Differentiation and Migration That Contributes to Neointimal Formation

Figure 3

Myogenic induction of BMSCs. (a) Immunofluorescence expressions of BMSCs. (A–C) BMSC treated with anti-α-SMA showing a blue-stained nucleus and a red-stained cytoplasm after coculture for 1, 3, or 7 days (×400); α-SMA expressions of BMSCs positive rates were 23% at 1 day, 49% at 3 days, or 82% at 7 days. (b) Expressions of α-SMA, TGF-β1, and Smad3 mRNA in BMSCs showing significant differences among the four groups were indicated by RT-PCR. (c) Results represent the average of at least six independent experiments (). Bars indicate the mean ± SD. compared to the control (model versus control, ; the model group for 3 days or 7 days versus 1 day, ).

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