Research Article
Hepatocyte Growth Factor Effects on Mesenchymal Stem Cells Derived from Human Arteries: A Novel Strategy to Accelerate Vascular Ulcer Wound Healing
Figure 3
Migratory effect of HGF/SF on hVW-MSC. (a) Crystal Violet staining of hVW-MSCs migrated into the scratch area after 24 hrs of exposure to HGF/SF in comparison to the untreated hVW-MSCs; the cell migration was slackened by PHA-665752 before treatment. Wounded area is delimited by black lines. Scale bars = 50 μm. (b) Quantitative analysis of cell migration area in hVW-MSCs treated with HGF/SF, in presence and absence of PHA-665752. (c) Immunofluorescence staining for Vimentin revealed that the intermediate filaments Vimentin (positive (green)) was markedly stained in migrated cells (arrows) then unmigrated cells. Scale bars = 100 μm. The reported images are representative of three independent experiments.
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