In Vitro Characterization of Human Mesenchymal Stem Cells Isolated from Different Tissues with a Potential to Promote Complex Bone Regeneration

Figure 3

The trilineage differentiation ability of MSC isolates. The expression level of the RUNX2 (a) and ALP (b) genes in the undifferentiated and differentiated samples, determined with RT-qPCR measurements. Alizarin red staining was used to demonstrate the calcium deposition of the matured bone matrix after 14 days of osteogenic induction (c). The stain was dissolved and quantified colorimetrically (d). Lipid accumulation was confirmed by oil red O staining (red dots) after adipogenic induction; cells were counterstained with dimethyl methylene blue stain (e). The concentration of the red stain was determined colorimetrically (f). The adipogenesis was followed with RT-qPCR measurement of PPARγ gene (g). Chondrogenic differentiation was confirmed in all samples (h). The photos demonstrate representative samples. value less than 0.001.

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