Research Article
In Vitro Characterization of Human Mesenchymal Stem Cells Isolated from Different Tissues with a Potential to Promote Complex Bone Regeneration
Figure 4
Endothelial differentiation potential of the MSCs was tested in Matrigel tube formation assay. Tubular-like structures were photographed (a) and analyzed with TubeCount software. Human umbilical vein endothelial cells (HUVEC) were used as controls. Total tube length (b) and numbers (c) were counted and presented in the diagrams. The changes in the expression of PECAM1 during endothelial differentiation were followed with RT-qPCR. Samples were collected from undifferentiated and predifferentiated states (7-day endothelial induction) and after angiogenesis in a Matrigel assay (d). The 3D network developed by a representative PDLSC sample was demonstrated with fluorescence staining of the cell membrane by DiSC3(5) (red) and the nucleus by DAPI (cyan) (e). The photos demonstrate representative samples. value less than 0.001.
| (a) |
| (b) |
| (c) |
| (d) |
| (e) |