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Stem Cells International
Volume 2016, Article ID 3816256, 11 pages
http://dx.doi.org/10.1155/2016/3816256
Research Article

Differentiation of Bone Marrow Mesenchymal Stem Cells to Cardiomyocyte-Like Cells Is Regulated by the Combined Low Dose Treatment of Transforming Growth Factor-β1 and 5-Azacytidine

1Emergency Center, Anzhen Hospital, Capital Medical University, Beijing 100029, China
2Beijing Institute of Heart Lung and Blood Vessel Diseases, Beijing 100029, China

Received 19 April 2015; Revised 24 May 2015; Accepted 15 July 2015

Academic Editor: Isotta Chimenti

Copyright © 2016 Shutian Shi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. A. S. Go, D. Mozaffarian, V. L. Roger et al., “Heart disease and stroke statistics—2014 Update: a report from the American Heart Association,” Circulation, vol. 129, no. 3, pp. e28–e292, 2014. View at Publisher · View at Google Scholar · View at Scopus
  2. N. J. Pagidipati and T. A. Gaziano, “Estimating deaths from cardiovascular disease: a review of global methodologies of mortality measurement,” Circulation, vol. 127, no. 6, pp. 749–756, 2013. View at Publisher · View at Google Scholar · View at Scopus
  3. C. Brenner and W.-M. Franz, “The use of stem cells for the repair of cardiac tissue in ischemic heart disease,” Expert Review of Medical Devices, vol. 8, no. 2, pp. 209–225, 2011. View at Publisher · View at Google Scholar · View at Scopus
  4. R. J. Dilley and W. A. Morrison, “Vascularisation to improve translational potential of tissue engineering systems for cardiac repair,” International Journal of Biochemistry and Cell Biology, vol. 56, pp. 38–46, 2014. View at Publisher · View at Google Scholar · View at Scopus
  5. H. K. Haider, S. A. Akbar, and M. Ashraf, “Angiomyogenesis for myocardial repair,” Antioxidants and Redox Signaling, vol. 11, no. 8, pp. 1929–1944, 2009. View at Publisher · View at Google Scholar · View at Scopus
  6. K. Malliaras and E. Marbán, “Cardiac cell therapy: where we've been, where we are, and where we should be headed,” British Medical Bulletin, vol. 98, no. 1, pp. 161–185, 2011. View at Publisher · View at Google Scholar · View at Scopus
  7. J. Kim, L. Shapiro, and A. Flynn, “The clinical application of mesenchymal stem cells and cardiac stem cells as a therapy for cardiovascular disease,” Pharmacology & Therapeutics, vol. 151, pp. 8–15, 2015. View at Publisher · View at Google Scholar
  8. A. Behfar, S. Yamada, R. Crespo-Diaz et al., “Guided cardiopoiesis enhances therapeutic benefit of bone marrow human mesenchymal stem cells in chronic myocardial infarction,” Journal of the American College of Cardiology, vol. 56, no. 9, pp. 721–734, 2010. View at Publisher · View at Google Scholar · View at Scopus
  9. S. Makino, K. Fukuda, S. Miyoshi et al., “Cardiomyocytes can be generated from marrow stromal cells in vitro,” The Journal of Clinical Investigation, vol. 103, no. 5, pp. 697–705, 1999. View at Publisher · View at Google Scholar · View at Scopus
  10. M. N. Duong, Y.-T. Ma, and R. C. J. Chiu, “Bone marrow stem cells,” Methods in Molecular Biology, vol. 1036, pp. 33–46, 2013. View at Publisher · View at Google Scholar · View at Scopus
  11. T.-S. Li, T. Komota, M. Ohshima et al., “TGF-β induces the differentiation of bone marrow stem cells into immature cardiomyocytes,” Biochemical and Biophysical Research Communications, vol. 366, no. 4, pp. 1074–1080, 2008. View at Publisher · View at Google Scholar · View at Scopus
  12. S. Mohanty, S. Bose, K. G. Jain, B. Bhargava, and B. Airan, “TGFβ1 contributes to cardiomyogenic-like differentiation of human bone marrow mesenchymal stem cells,” International Journal of Cardiology, vol. 163, no. 1, pp. 93–99, 2013. View at Publisher · View at Google Scholar · View at Scopus
  13. Y. Xing, A. Lv, L. Wang, X. Yan, W. Zhao, and F. Cao, “Engineered myocardial tissues constructed in vivo using cardiomyocyte-like cells derived from bone marrow mesenchymal stem cells in rats,” Journal of Biomedical Science, vol. 19, article 6, 2012. View at Publisher · View at Google Scholar · View at Scopus
  14. Q. Gao, M. Guo, X. Jiang, X. Hu, Y. Wang, and Y. Fan, “A cocktail method for promoting cardiomyocyte differentiation from bone marrow-derived mesenchymal stem cells,” Stem Cells International, vol. 2014, Article ID 162024, 11 pages, 2014. View at Publisher · View at Google Scholar · View at Scopus
  15. X. Liu, J. Wang, X. Ji, S. Yu, and L. Wei, “Preconditioning of bone marrow mesenchymal stem cells by prolyl hydroxylase inhibition enhances cell survival and angiogenesis in vitro and after transplantation into the ischemic heart of rats,” Stem Cell Research & Therapy, vol. 5, no. 5, p. 111, 2014. View at Publisher · View at Google Scholar
  16. J. M. Hare, J. H. Traverse, T. D. Henry et al., “A randomized, double-blind, placebo-controlled, dose-escalation study of intravenous adult human mesenchymal stem cells (prochymal) after acute myocardial infarction,” Journal of the American College of Cardiology, vol. 54, no. 24, pp. 2277–2286, 2009. View at Publisher · View at Google Scholar · View at Scopus
  17. J.-W. Lee, S.-H. Lee, Y.-J. Youn et al., “A randomized, open-label, multicenter trial for the safety and efficacy of adult mesenchymal stem cells after acute myocardial infarction,” Journal of Korean medical science, vol. 29, no. 1, pp. 23–31, 2014. View at Publisher · View at Google Scholar · View at Scopus
  18. B. Liu, C.-Y. Duan, C.-F. Luo et al., “Effectiveness and safety of selected bone marrow stem cells on left ventricular function in patients with acute myocardial infarction: a meta-analysis of randomized controlled trials,” International Journal of Cardiology, vol. 177, no. 3, pp. 764–770, 2014. View at Publisher · View at Google Scholar · View at Scopus
  19. S. J. Greco, K. Liu, and P. Rameshwar, “Functional similarities among genes regulated by OCT4 in human mesenchymal and embryonic stem cells,” Stem Cells, vol. 25, no. 12, pp. 3143–3154, 2007. View at Publisher · View at Google Scholar · View at Scopus
  20. F. Z. Asumda and P. B. Chase, “Age-related changes in rat bone-marrow mesenchymal stem cell plasticity,” BMC Cell Biology, vol. 12, article 44, 2011. View at Publisher · View at Google Scholar · View at Scopus
  21. T.-L. Yew, F.-Y. Chiu, C.-C. Tsai et al., “Knockdown of p21Cip1/Waf1 enhances proliferation, the expression of stemness markers, and osteogenic potential in human mesenchymal stem cells,” Aging Cell, vol. 10, no. 2, pp. 349–361, 2011. View at Publisher · View at Google Scholar · View at Scopus
  22. D. Bhartiya, “Are mesenchymal cells indeed pluripotent stem cells or just stromal cells? OCT-4 and VSELs biology has led to better understanding,” Stem Cells International, vol. 2013, Article ID 547501, 6 pages, 2013. View at Publisher · View at Google Scholar · View at Scopus
  23. H. S. Kim, J. W. Cho, K. Hidaka, and T. Morisaki, “Activation of MEK-ERK by heregulin-beta1 promotes the development of cardiomyocytes derived from ES cells,” Biochemical and Biophysical Research Communications, vol. 361, no. 3, pp. 732–738, 2007. View at Publisher · View at Google Scholar · View at Scopus
  24. Q. Qian, H. Qian, X. Zhang et al., “5-Azacytidine induces cardiac differentiation of human umbilical cord-derived mesenchymal stem cells by activating extracellular regulated Kinase,” Stem Cells and Development, vol. 21, no. 1, pp. 67–75, 2012. View at Publisher · View at Google Scholar · View at Scopus
  25. K. Fukuda, “Regeneration of cardiomyocytes from bone marrow: use of mesenchymal stem cell for cardiovascular tissue engineering,” Cytotechnology, vol. 41, no. 2-3, pp. 165–175, 2003. View at Publisher · View at Google Scholar · View at Scopus
  26. S.-C. Lien, S. Usami, S. Chien, and J.-J. Chiu, “Phosphatidylinositol 3-kinase/Akt pathway is involved in transforming growth factor-β1-induced phenotypic modulation of 10T1/2 cells to smooth muscle cells,” Cellular Signalling, vol. 18, no. 8, pp. 1270–1278, 2006. View at Publisher · View at Google Scholar · View at Scopus