PS1/γ-Secretase-Mediated Cadherin Cleavage Induces β-Catenin Nuclear Translocation and Osteogenic Differentiation of Human Bone Marrow Stromal Cells

Figure 2

Wnt3a-CM stimulates proliferation and impairs BMSCs differentiation. (a-b) Cumulative population doublings of BMSCs cultured with either expansion medium (EM (a)) or incomplete osteogenic medium (iOM, without dexamethasone (b)) supplemented with 1% or 10% Wnt3a-CM. Data represent mean ± SD of 3 independent experiments with triplicates. (c-d) BMSCs expanded in the presence of 10% Wnt3a-CM for 10 days were plated at clonal density and maintained in control expansion medium for another 14 days. Morphology (c) and quantification (d) of CFU-F formed by control (CT) and Wnt3a-expanded BMSCs are shown. Data represent mean ± SD of 4 experiments with quadruplicates. (e) Representative micrographs of control and Wnt3a-expanded BMSCs induced towards the osteogenic (left panel) and adipogenic lineages (right panel) stained with Von Kossa and Oil Red O, respectively. Bars = 100 μm. (f) Quantification of total mineralized area and fat accumulating cells per field of view. Data represent mean ± SD of 4 experiments with triplicates. ; .

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