PS1/γ-Secretase-Mediated Cadherin Cleavage Induces β-Catenin Nuclear Translocation and Osteogenic Differentiation of Human Bone Marrow Stromal Cells

Figure 4

PS1/γ-secretase-mediated cadherin cleavage occurs during osteogenic differentiation of BMSCs, generating a C-terminal intracellular fragment (CTF-2) that translocates to nucleus. BMSCs were osteoinduced for 48 h in the presence of Dapt. (a) Representative immunoblotting showing a full-length cadherin band (135 kDa) and its cleavage products (CTF-1 and CTF-2) in total protein extracts. (b) Densitometric quantification of the 135 kDa cadherin band. Bars show mean ± SD of 4 independent experiments. (c) Representative immunoblotting showing CTF-2 fragments (35 kDa) in cytoplasmic and nuclear fractions. (d) Quantification of nuclear CTF-2 in 3 experiments. CT = control undifferentiated cells; OM = osteogenic medium; Dapt + OM = osteogenic medium with Dapt. relative to CT.

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