In Vivo Tracking of Systemically Administered Allogeneic Bone Marrow Mesenchymal Stem Cells in Normal Rats through Bioluminescence Imaging

Figure 1

Characterization of rats treated with Luc-GFP-BMSCs. (a) GFP expression in Luc-GFP-BMSCs. The transduction efficiency was estimated by comparing the number of GFP-positive cells with the total cells via Hoechst staining. (b and c) The capability of Luc-GFP-BMSCs to differentiate according to an adipocyte or osteopenia pattern was verified by staining with Oil Red O and Alizarin red, respectively. After adipocyte and osteopenia differentiation, the cells still express GFP. (d) BLI of varying numbers of Luc-GFP-BMSCs in vitro. Representative images of at least three independent experiments. (e) Quantitative analysis revealed a strong linear relationship between cell numbers and the BLI signal ().

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