In Vivo Tracking of Systemically Administered Allogeneic Bone Marrow Mesenchymal Stem Cells in Normal Rats through Bioluminescence Imaging

Figure 2

BLI of the transplanted Luc-GFP-BMSCs in vivo. (a and c) Luc-GFP-BMSCs suspended in PBS were injected into normal rats, while PBS alone was injected as a control. Cells were imaged at different time points, showing initial detention in the lungs and gradual concentration in the back, demonstrating that BMSCs may possess a tissue-specific binding capacity for some organs. However, the signals disappeared at day 14, revealing the final loss of transplanted BMSCs in normal rats. (b and d) Quantitative analysis of BLI signals in the chest and back. Bioluminescence activity steadily decreased over time in the chest. However, in the back, significant enhancement occurred at one and seven days after injection. versus BLI signals in the chest at 1.5 h (); versus BLI signals in the back at 1.5 h ().