Absence of Rybp Compromises Neural Differentiation of Embryonic Stem Cells

Figure 4

Rybp deficient cells show impaired protein expression of neural markers during in vitro neural differentiation. Immunocytochemical analysis of in vitro differentiated neural cultures shows that there are more Nestin (a) and Pax6 (b) and less Tuj1 (c) are visible in the rybp null mutant neural cell cultures by d10. Nestin and Tuj1 staining are reduced in the cell culture by d14 compared to wild type, which indicates possible defects in the late neural processes. Immunostaining of astrocyte marker, Gfap (d), shows a small amount of astrocyte-like cells in wild type culture, which is absent in the mutant. Immunocytochemistry with antibody against Plagl1 (e) reveals that Plagl1 is expressed in the nucleus of cells but it is absent in cells. Magnification is 60x. Scale bars represent 60 μm.

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