Identification of CHD1L as an Important Regulator for Spermatogonial Stem Cell Survival and Self-Renewal

Figure 1

Expression of Chd1l in mouse testes. (a) Chd1l mRNA profiling in developing and adult mouse tissues, as assessed by quantitative RT-PCR analysis. Data presented here are mean ± SD of three animals (). (versus adult tissues). (b) Expression of Chd1l in developing mouse testis. Data presented here are mean ± SD of three developing mouse testes (). (versus 5-day testes). (c) Protein expression levels of CHD1L, GFRΑ1, and PLZF in developing mouse testis. Total proteins were harvested and subjected to Western Blot analyses. Representative images (left) and quantitative data (right) from three independent experiments () were presented here. (d) Immunohistochemistry assays show that CHD1L is mainly expressed in the spermatogonia of the developing mouse testis at 5 dpp, 10 dpp, 28 dpp, and 56 dpp. Long arrows indicate the positive (brown) staining in testis sections, and the arrowheads indicate the positive nucleus of Sertoli cells in 56 dpp testis sections. Scale bars represent 100 μm. Insets are magnified views. Representative images from three animals () were presented here. Quantitative RT-PCR: quantitative reverse transcription-polymerase chain reaction; CHD1L: chromodomain helicase/ATPase DNA binding protein 1-like gene; PLZF: promyelocytic leukemia zinc finger; GFRΑ1: GDNF-family receptor α-1; SD: standard deviation; dpp: days postpartum.

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