Identification of CHD1L as an Important Regulator for Spermatogonial Stem Cell Survival and Self-Renewal

Figure 2

CHD1L is coexpressed with other SSC markers in undifferentiated spermatogonia cells. Immunofluorescence staining of mouse testicular tissues harvested at 5 dpp, sectioned, and subjected to immunofluorescence staining with antibodies against CHD1L (red), PLZF (green), GFRΑ1 (green), or GFP (green), respectively. The nuclei were visualized with DAPI (blue). The right panels are the merged images showing coexpression of CHD1L with PLZF, GFRΑ1, or GFP in most undifferentiated spermatogonia at 5 dpp. Normal goat or rabbit IgG were applied as negative controls (bottom row). Representative images from three animals () were presented here. Scale bars represent 50 μm. SSC: spermatogonial stem cell; dpp: days postpartum; PLZF: promyelocytic leukemia zinc finger; GFRΑ1: GDNF-family receptor α-1; DAPI: 4′,6-diamidino-2-phenylindole.