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Stem Cells International
Volume 2016, Article ID 4069543, 12 pages
Research Article

Identification of CHD1L as an Important Regulator for Spermatogonial Stem Cell Survival and Self-Renewal

Department of Histology and Embryology, Guangzhou Medical University, Guangzhou, China

Received 28 September 2016; Accepted 27 October 2016

Academic Editor: Qingzhong Xiao

Copyright © 2016 Shan-Shan Liu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Table 1: Primers used for quantitative RT-PCR. Supplementary Table 2: Antibodies used in experiment. Supplementary S1: Flow cytometric analysis of GFRα1 expression CD90+ SSCs. The undifferentiated spermatogonial stem cells were isolated from mouse testis at 5 dpp by using magnetic-activated cell sorting with CD90 conjugated microbeads. Isolated CD90+ cells were subjected to flow cytometry analyses using antibody against GFRα1 to examine GFRα1 expression on CD90+ SSCs. Left, CD90+ cells were gated and counted (green dot). Right, GFRα1 expression in CD90+ SSCs.

  1. Supplementary Material